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The microbial growth curve is a curve with the number of microorganisms (the number of living bacteria or the weight of bacteria) as the ordinate and the incubation time as the abscissa. Generally speaking, the change in the weight of microorganisms (bacteria) can more essentially reflect the growth process than the change in the number. The curve can be divided into three stages, namely, the stage of increasing growth rate (logarithmic growth stage), the stage of decreasing growth rate, and the endogenous respiration stage. The microbial growth curve analyzer uses the turbidimetric method, that is, a method in which the growth of microorganisms causes an increase in the turbidity of the culture. Measurement values are taken at intervals to draw the microbial growth curve. This product integrates the shaking culture of microorganisms with the high - precision temperature control function of the computer, so as to monitor the growth status of various microorganisms in real - time.
Traditional turbidimetric method
(Determination of a single microbial strain)
1.Preparation of seed liquid: Take 1 vial of bacterial strain and inoculate it into the culture medium. Incubate for 18 hours to obtain the seed culture liquid.
2.Labeling and numbering: Prepare 48 Erlenmeyer flasks or test tubes filled with sterile culture medium and number them from 0 to 48 respectively.
3.Inoculation and culture: Pipette 1 mL of the seed liquid into each of the 48 numbered Erlenmeyer flasks or test tubes, and culture with shaking at 37 °C.
4.Measurement of growth amount: Every half an hour, take out the corresponding Erlenmeyer flask or test tube, pour the content into a cuvette, and place it in a spectrophotometer to measure the OD value. 5.Analysis of experimental results: Input the measured OD values into software to draw and analyze the growth curve of this bacterial strain.
Automatic Microbial Growth Curve Analyzer
(Simultaneous determination of 1 - 192 microbial strains)
1. Preparation of seed liquid: Take 1 - 192 vials of bacterial strains and inoculate them into the culture medium. Incubate for 18 hours to obtain the seed culture liquid.
2. Inoculation: Add 1 - 192 microbial solutions to a 48 - well plate and place it into the GCA - 2000.
3. Automated result analysis: Every half an hour, the GCA - 2000 will measure 1 - 192 sample microbial solutions and draw the growth curves in real - time.
Through the above comparison, it can be clearly seen that the automatic microbial growth curve analyzer has significant advantages over the traditional turbidimetric method in terms of the ability to handle multiple microbial strains, the degree of automation, and efficiency. It is especially applicable to scenarios where the growth situations of a large number of microbial strains need to be analyzed simultaneously, while the traditional turbidimetric method still has its application value in the fine determination of a single microbial strain.
Key Advantages
Time-saving:
Up to 192 microbial samples can be monitored at one time, and the growth curves of each sample can be generated.
Labor-saving:
It features fully automatic online timed detection, eliminating the need for regular sampling.
Cost-saving:
It can save the consumption of reagents, consumables, etc., thus reducing the experimental costs.
Accurate:
The analysis and comparison of the growth curves of up to 192 microorganisms can be carried out simultaneously, eliminating various influencing factors between different batches.
Application Field
The Automatic Microbial Growth Curve Analyzer is applied in the following fields:
1.Influences of Different Inorganic and Organic Substances on 2.Microbial Growth
3.Screening of Superior Microbial Strains
4.Research on Microbial Mechanisms
5.Analysis of Growth Kinetic Curves
6.Determination of Food Safety Microorganisms
7.Research and Development of Antimicrobial Agents
8.Improvement of Microbial Fermentation Processes
9.Determination of Endotoxins
10.Synthetic Biology
11.Screening of Culture Media
12.Detection of Pathogenic Bacteria
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Model |
GCA-2000H |
GCA-2000R |
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LED Light Source Lifespan |
25,000 hours |
25,000 hours |
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Number of Channels |
192 |
192 |
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Types of Adaptable Microorganisms |
Anaerobic and Aerobic Microorganisms |
Anaerobic and Aerobic Microorganisms |
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Detectable Time Interval |
Adjustable from 5 to 360 minutes |
4-60℃ |
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OD600 Range |
0-5 |
0-5 |
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Temperature Control Range |
Room Temperature + 5 - 60 °C |
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Shaking Speed |
0-1000rpm/min |
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Cultivation Volume |
0.2-4ml |
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Cultivation Time |
0 - 30 days |
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Chief Scientist
Professor Mori has been engaged in research on systems microbiology and synthetic biology for over thirty years. He is an internationally recognized top scientist in this field and is regarded as the founder of the discipline of systems microbiology in Japan. He has undertaken the major research work on the bacterial genome part in the Human Genome Project. He has also taken on more than twenty major special projects in frontier fields and basic sciences in Japan as well as international cooperation projects.
He has published 163 academic papers in international top-tier journals such as Nature, Science, Nature Biotechnology, and Nature Methods. The highest impact factor of a single paper has reached 41.8, and a single paper has been cited as many as 6,798 times. More than 40 papers have been cited over 100 times. He has developed six sets of Escherichia coli whole-genome knockout libraries, providing more than 300,000 strains of Escherichia coli gene mutant strains to scientific research teams in over a dozen countries and regions around the world.
He has also provided key technical support and been commissioned to develop important products for well-known multinational enterprises such as Ajinomoto and GlaxoSmithKline plc.
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