Shenzhen East Scientific Instrument Co., Ltd.

Traditional Method (Repeating for Each Microbial Strain)

Strain Preparation: According to the requirements of the experiment, take N vials of bacterial strains, which can be preserved in either liquid or solid culture media. This step serves as the foundation for subsequent operations, as the quality and quantity of the strains directly affect the experimental results.

Labeling and Numbering: Meticulously prepare N test tubes filled with culture media that have undergone strict sterilization treatment, and number each test tube sequentially from 1 to N. Accurate numbering helps with subsequent tracking and distinguishing of each microbial strain, avoiding confusion.

Aseptic Inoculation: In the strictly aseptic environment of a laminar flow cabinet, use an inoculating loop to carefully scrape a small amount of colonies from the original microbial strain and then inoculate them into the numbered test tubes. This step requires extremely precise operation, as any slight contamination may lead to the failure of the experiment, since it directly introduces the target microbial strain into a new culture environment.

Low-Temperature Preservation: Place the inoculated test tubes into an ultra-low-temperature freezer and store them according to the corresponding numbers. The ultra-low-temperature environment can effectively inhibit the metabolic activities of microorganisms, prolong the preservation time of the strains, and at the same time ensure that the viability of the strains remains unaffected, for subsequent use in the experiment. During the operation process of this traditional method, each step needs to be carefully completed by hand. Although it can ensure the accuracy of inoculation for each microbial strain, it has a relatively low efficiency in handling a large number of strains and is prone to introducing errors due to human operational mistakes. Fully Automatic Microbial Colony Workstation (Capable of Completing 96 - 6144 Inoculations in a Single Program Run)

Strain Preparation: Place the culture plate carrying the mother strain and the subculture plate for subsequent cultivation accurately in the positions designated by the machine in advance. This step is relatively simple, only requiring that the culture plates be placed correctly to prepare for subsequent automated inoculation.

Programmed Inoculation: By setting specific programs, complex operations such as replication, combination, and dispersion of the microbial strains can be achieved. This automated programmed inoculation method significantly improves the inoculation efficiency and accuracy, enabling the handling of a large number of strain inoculation tasks within a short period of time while avoiding the errors that may occur during manual inoculation.

Low-Temperature Preservation: Place the inoculated culture plates into an ultra-low-temperature freezer for preservation. Similar to the traditional method, the ultra-low-temperature environment can maintain the viability and stability of the microbial strains, reserving samples for subsequent experiments or research.

The automatic microbial colony workstation is applied in the following fields:

Full Automation: The automatic microbial colony workstation enables automated inoculation. Users only need to program the automatic microbial colony workstation according to the experimental design.

Ultra-High Precision: The automatic microbial colony workstation can precisely transfer between 96, 384, 1536, and 6144 colony array culture plates.

Time-Saving: With the operation of the ultra-high-speed magnetic levitation motor, the automatic microbial colony workstation can complete the operation of 100 culture plates within one hour.

Labor-Saving: The automatic microbial colony workstation can be automatically completed just by programming it according to the experiment, eliminating the need for substantial labor operation costs.